Key aspects in the development of the central nervous system (CNS) include the formation of neuronal axons, their subsequent growth and guidance through thick layers of nervous tissue, and the folding of the brain. We are taking an interdisciplinary approach and investigate how cellular forces, local cell and tissue compliance and cellular mechanosensitivity contribute to CNS development and disease. Methods we are exploiting include atomic force microscopy, traction force microscopy, custom-built simple and complex compliant cell culture substrates, optical microscopy including confocal laser scanning microscopy and cell biological techniques.